įood authentication is one of the critical issues in food processing, which verifies that a food is in compliance with legal requirements of label. Design and Validation of Short-Amplicon Length PCR Assay for the Detection of Porcine Gelatin in Commercial Candy and Marshmallow Products. Keywords:Ĭandy Conventional PCR Gelatin, Marshmallow Porcine Species-Specific Primers So, we demonstrated that the authentication of commercial sweet ingredients using PCR assay is effective and protect consumers from being mislead due to mislabels. Nine of the positive samples were Gelatin labeled, and eight were not identified to contain gelatin. The results revealed that seventeen samples were found to contain porcine gelatin ingredients and the rest 86 were found to be negative. One hundred and three samples including seventy six samples from candies and twenty seven samples from marshmallow were purchased from local market in Amman, Jordan. A pair of porcine-specific primers defining a 74 bp region of the mitochondrial D-loop sequence were designed. In this study, Porcine-specific short amplicon-size for conventional PCR assay was developed for the detection of gelatin in commercial candy and marshmallow products. To ensure the compliance of food products with halal regulations and label description, reliable analytical methods are required.
PS gelatin suppressed proliferation of spleen cells and of RL male symbol 1 cells without FCS.Porcine gelatin has been used in many food products. In both cases, fetal calf serum (FCS) was required. In conclusion, BB gelatin enhanced proliferation of spleen cells and suppressed proliferation of RL male symbol 1 cells. Enhancement of spleen cell proliferation by BB gelatin was time-dependent but suppression of RL male symbol 1 cell proliferation by PS gelatin was not. Inhibition of RL male symbol 1 cell proliferation by PS gelatin was not affected by BB gelatin, but enhancement of spleen cell proliferation by BB gelatin was attenuated by PS gelatin regardless of the sequence of treating the spleen cells with PS gelatin. MMC-treated RL male symbol 1 cells as well as MMC-treated spleen cells suppressed the proliferation of spleen cells augmented by BB gelatin. Mitomycin C (MMC)-treated spleen cells as well as MMC-treated RL male symbol 1 cells partly released RL male symbol 1 cells from the inhibition of proliferation by PS gelatin. BB gelatin as well as PS gelatin suppressed proliferation of RL male symbol 1 cells, a T cell lymphoma cell line of Balb/c mice, and such an activity of BB gelatin was not exerted in the serum-free medium whereas PS gelatin exerted its activity in the same medium. It is not known whether or not such an activity of PS gelatin on Con A-stimulated spleen cells is exerted in the serum-free medium since Con A was unable to augment proliferation of spleen cells in that medium. On the other hand, PS gelatin suppressed proliferation of normal spleen cells and of those stimulated by concanavalin A (Con A). The present study showed that such an activity of BB gelatin was not exerted in a serum-free medium. We previously observed that BB gelatin enhanced spleen cell proliferation.
#Beef or porcine gelatin skin
We investigated relationship between porcine skin (PS) and bovine bone (BB) gelatins in their actions on proliferation of murine benign and malignant cells in this study.
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